Format

Send to

Choose Destination
See comment in PubMed Commons below
Diagn Microbiol Infect Dis. 2005 Oct;53(2):121-4. Epub 2005 Sep 15.

Rapid, inexpensive MIC determination of Mycobacterium tuberculosis isolates by using microplate nitrate reductase assay.

Author information

  • 1Biotechnology Division, Regional Research Laboratory, Jammu-Tawi 180001, India.

Abstract

A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center