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J Food Prot. 2005 Sep;68(9):1907-10.

Multiplex PCR assay simplifies serotyping and sequence typing of Listeria monocytogenes associated with human outbreaks.

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Department of Food Science, Pennsylvania State University, University Park, Pennsylvania 16802-2504, USA.


Listeria monocytogenes serotypes 1/2a and 4b are responsible for the majority of cases of human listeriosis worldwide. In this study, a multiplex PCR assay was developed to allow rapid identification and easily interpretable differentiation of serotypes 1/2a and 4b from other serotypes of L. monocytogenes by simultaneously targeting two virulence genes (inlB and inlC) and two serotype-specific genes (ORF2372 and Imo0171). A subsequent gel extraction and sequence typing analysis of the highly polymorphic intragenic regions in inlB and inlC simplified a previously developed multi-virulence-locus sequence typing scheme and provided discriminatory power for subtyping L. monocytogenes similar to pulsed-field gel electrophoresis analysis.

[Indexed for MEDLINE]

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