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BMC Genomics. 2005 Sep 12;6:118.

Rapid single nucleotide polymorphism mapping in C. elegans.

Author information

1
Department of Biology, University of Utah, Salt Lake City, Utah 84112-0840, USA. wdavis@biology.utah.edu

Abstract

BACKGROUND:

In C. elegans, single nucleotide polymorphisms (SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes.

RESULTS:

Here, we describe a set of 48 primer pairs that flank SNPs evenly spaced across the C. elegans genome and that work under identical PCR conditions. Each SNP in this set alters a DraI site, enabling rapid and parallel scoring. We describe a procedure using these reagents to quickly and reliably map mutations. We show that these techniques correctly map a known gene, dpy-5. We then use these techniques to map mutations in an uncharacterized strain, and show that its behavioral phenotype can be simultaneously mapped to three loci.

CONCLUSION:

Together, the reagents and methods described represent a significant advance in the accurate, rapid and inexpensive mapping of genes in C. elegans.

PMID:
16156901
PMCID:
PMC1242227
DOI:
10.1186/1471-2164-6-118
[Indexed for MEDLINE]
Free PMC Article

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