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Nat Biotechnol. 2005 Oct;23(10):1308-14. Epub 2005 Sep 11.

Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity.

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1
Department of Pharmacology, University of California, San Diego, 9500 Gilman Dr., La Jolla, California, 92093-0647, USA.

Abstract

Membrane-permeant biarsenical dyes such as FlAsH and ReAsH fluoresce upon binding to genetically encoded tetracysteine motifs expressed in living cells, yet spontaneous nonspecific background staining can prevent detection of weakly expressed or dilute proteins. If the affinity of the tetracysteine peptide could be increased, more stringent dithiol washes should increase the contrast between specific and nonspecific staining. Residues surrounding the tetracysteine motif were randomized and fused to GFP, retrovirally transduced into mammalian cells and iteratively sorted by fluorescence-activated cell sorting for high FRET from GFP to ReAsH in the presence of increasing concentrations of dithiol competitors. The selected sequences show higher fluorescence quantum yields and markedly improved dithiol resistance, culminating in a >20-fold increase in contrast. The selected tetracysteine sequences, HRWCCPGCCKTF and FLNCCPGCCMEP, maintain their enhanced properties as fusions to either terminus of GFP or directly to beta-actin. These improved biarsenical-tetracysteine motifs should enable detection of a much broader spectrum of cellular proteins.

PMID:
16155565
DOI:
10.1038/nbt1136
[Indexed for MEDLINE]
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