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Appl Environ Microbiol. 2005 Sep;71(9):5614-8.

Improved system for construction and analysis of single-copy beta-galactosidase operon fusions in Yersinia enterocolitica.

Author information

1
Department of Microbiology MSB 228, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.

Abstract

We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous beta-galactosidase activity.

PMID:
16151161
PMCID:
PMC1214615
DOI:
10.1128/AEM.71.9.5614-5618.2005
[Indexed for MEDLINE]
Free PMC Article

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