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Chembiochem. 2005 Oct;6(10):1899-905.

Probing lectin and sperm with carbohydrate-modified quantum dots.

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  • 1Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.

Abstract

We report the encapsulation of quantum dots with biologically important beta-N-acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc-encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose-encapsulated QDs (QDMANs) with sperm revealed site-specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.

PMID:
16149042
DOI:
10.1002/cbic.200500112
[PubMed - indexed for MEDLINE]
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