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Clin Diagn Lab Immunol. 2005 Sep;12(9):1127-9.

Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09).

Author information

1
University of Vermont College of Medicine, Burlington, VT 05405. beth.kirkpatrick@uvm.edu

Abstract

Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at <or=42 spots/10(6) peripheral blood lymphocytes.

PMID:
16148184
PMCID:
PMC1235803
DOI:
10.1128/CDLI.12.9.1127-1129.2005
[Indexed for MEDLINE]
Free PMC Article

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