Detection of aerosolized Alternaria alternata conidia, hyphae, and fragments by using a novel double-immunostaining technique

Clin Diagn Lab Immunol. 2005 Sep;12(9):1114-6. doi: 10.1128/CDLI.12.9.1114-1116.2005.

Abstract

A double-immunostaining halogen immunoassay was developed to identify aerosolized conidia, hyphae, and fragments of Alternaria alternata by using an anti-Alternaria polyclonal antiserum, while, simultaneously, allergy to these components was concurrently determined by using human immunoglobulin E antibodies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aerosols
  • Alternaria / immunology*
  • Antibodies, Fungal / immunology
  • Antigens, Fungal / analysis*
  • Antigens, Fungal / immunology
  • Halogens
  • Humans
  • Hypersensitivity / diagnosis
  • Hypersensitivity / immunology
  • Hyphae / immunology
  • Immunoassay / methods*
  • Immunoglobulin E / immunology
  • In Vitro Techniques
  • Mycoses / diagnosis
  • Mycoses / immunology*

Substances

  • Aerosols
  • Antibodies, Fungal
  • Antigens, Fungal
  • Halogens
  • Immunoglobulin E