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Cell Mol Life Sci. 2005 Oct;62(19-20):2350-8.

Caffeine intake induces an alteration in human neutrophil A2A adenosine receptors.

Author information

1
Department of Clinical and Experimental Medicine, Pharmacology Unit, via Fossato di Mortara 17-19, 44100, Ferrara, Italy.

Abstract

Caffeine is the most widely used drug in the world and acts mainly through antagonism of the effects mediated by the adenosine receptor subtypes A1, A2A, A2B and A3. We determined whether repeated caffeine administration at different doses and for different periods of time (400 or 600 mg/day for 1 week and 400 mg/day for 2 weeks) alters human neutrophil A2A adenosine receptor density and function. Saturation binding assays showed an increase in affinity (K(D)) and density (B(max)) of A2A adenosine receptors after caffeine intake. These changes were accompanied by increases in cAMP accumulation and decreases in superoxide anion production after stimulation of the A2A receptor subtype using the agonist 5'-N-ethylcarboxamidoadenosine (NECA). Binding and functional changes of A2A receptors returned to baseline after 48 h of caffeine withdrawal. The findings are consistent with a potential anti-inflammatory effect of caffeine mediated by neutrophil A2A receptors.

PMID:
16143823
DOI:
10.1007/s00018-005-5312-z
[Indexed for MEDLINE]

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