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Vaccine. 2006 Jan 9;24(1):82-9. Epub 2005 Aug 11.

Expressions of Bovine IFN-gamma and foot-and-mouth disease VP1 antigen in P. pastoris and their effects on mouse immune response to FMD antigens.

Author information

1
College of Veterinary Medicine, No. 2, yuanmingyuan west Road, haidian District 100094, Beijing, PR China. shixj@bjciq.gov.cn

Abstract

As a highly contagious disease in cloven-hoofed animals, foot-and-mouth disease virus (FMDV) may cause a considerable social-economic loss in those countries affected. IFN-gamma has a wide range of antiviral and immune modulating functions. Thus, to study the immune enhancing effects of recombinant Bovine IFN-gamma (rBoIFN-gamma) on a recombinant FMDV vaccine, BoIFN-gamma, FMDV VP1 and BoIFN-gamma/VP fusion genes were cloned, expressed, co-expressed in pichia pastoris (P. pastoris) respectively, and subsequent immune effects have been evaluated in this study. The results showed that the genes encoding for BoIFN-gamma, VP1 and BoIFN-gamma/VP1 are successfully expressed in P. pastoris and their products are directly secreted into the cultural supernatant at a high level of 1.0 g/L analyzed by thin-layer scanning. In addition, rVP1 alone could induce both humoral and marginal cell-mediated immune responses in mice, while the group with co-inoculations of rBoIFN-gamma could markedly enhance both humoral and cell-mediated immune responses; even more dramatic immune responses were observed with the group inoculated with the fusion product, rBoIFN-gamma/VP1. The fusion product could be further investigated for its utility of FMDV vaccine development.

PMID:
16135391
DOI:
10.1016/j.vaccine.2005.07.051
[Indexed for MEDLINE]

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