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Opt Lett. 2005 Aug 15;30(16):2134-6.

Structure sensitivity in third-harmonic generation microscopy.

Author information

1
Laboratory for Optics and Biosciences, Centre National de la Recherche Scientifique, Ecole Polytechnique, 91128 Palaiseau, France.

Abstract

We characterize experimentally the influence of sample structure and beam focusing on signal level in third-harmonic generation (THG) microscopy. In the case of a homogeneous spherical sample, the dependence of the signal on the size of the sphere can be controlled by modifying the Rayleigh length of the excitation beam. More generally, the influence of excitation focusing on the signal depends on sample geometry, allowing one to highlight certain structures within a complex system. We illustrate this point by focusing-based contrast modulation in THG images of Drosophila embryos.

PMID:
16127934
[Indexed for MEDLINE]

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