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Nat Biotechnol. 2005 Sep;23(9):1159-69. Epub 2005 Aug 28.

Production of human monoclonal antibody in eggs of chimeric chickens.

Author information

1
Origen Therapeutics, 1450 Rollins Road, Burlingame, California 94010, USA.

Abstract

The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and downstream DNA sequences of ovalbumin, a protein produced exclusively in very high quantities in chicken egg white, to drive tissue-specific expression of human mAb in chicken eggs. To accommodate these large regulatory regions, we established and transfected lines of chicken embryonic stem (cES) cells and formed chimeras that express mAb from cES cell-derived tubular gland cells. Eggs from high-grade chimeras contained up to 3 mg of mAb that possesses enhanced antibody-dependent cellular cytotoxicity (ADCC), nonantigenic glycosylation, acceptable half-life, excellent antigen recognition and good rates of internalization.

PMID:
16127450
DOI:
10.1038/nbt1132
[Indexed for MEDLINE]
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