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Appl Biochem Biotechnol. 2005 Aug;126(2):79-92.

Surface plasmon resonance: applications in understanding receptor-ligand interaction.

Author information

1
Virology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002 India. ppat@rediffmail.com

Abstract

During last decade there has been significant progress in the development of analytical techniques for evaluation of receptor-ligand interaction. Surface plasmon resonance (SPR)-based optical biosensors are now being used extensively to define the kinetics of wide variety of macromolecular interactions and high- and low-affinity small molecule interactions. The experimental design data analysis methods are evolving along with widespread applications in ligand fishing, microbiology, virology, host-pathogen interaction, epitope mapping, and protein-, cell-, membrane-, nucleic acid-protein interactions. SPR-based biosensors have strong impact on basic and applied research significantly. This brief review describes the SPR technology and few of its applications in relation to receptor-ligand interaction that has brought significant change in the methodology, analysis, interpretation, and application of the SPR technology.

PMID:
16118464
[Indexed for MEDLINE]

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