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Methods Mol Biol. 2006;313:107-20.

Yeast transformation by the LiAc/SS Carrier DNA/PEG method.

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1
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Canada.

Abstract

The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.

PMID:
16118429
DOI:
10.1385/1-59259-958-3:107
[Indexed for MEDLINE]
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