Format

Send to

Choose Destination
Methods Mol Biol. 2006;313:33-9.

Isolation of yeast mitochondria.

Author information

1
Institut für Biochemie und Molekularbiologie, Universität Freiburg, Germany.

Abstract

Often preparations of isolated organelles contain other, unwanted, cellular components. For biochemical experiments to determine the localization of newly identified proteins, or to determine the whole set of proteins (or the proteome) from a desired organelle, these unwanted components often confuse the resulting data. For these types of studies, it is crucial to have highly pure fractions of the desired organelle. Here we describe a protocol for purification of mitochondria from Saccharomyces cerevisiae cells devoid of contamination from other cellular compartments.

PMID:
16118422
DOI:
10.1385/1-59259-958-3:033
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center