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[Establishment and judgement of the animal model of focal ischemia-reperfusion].

[Article in Chinese]

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1
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China.

Abstract

OBJECTIVE:

To investigate the influence of reperfusion after ischemia for 90, 120, 180 minutes on the focal ischemia reperfusion animal model.

METHODS:

The string-fastening method was used to establish focal ischemia-reperfusion models in SD rats and the rats were divided into 4 groups: ischemia-reperfusion for 90, 120 and 180 minute groups and the sham operation group. TTC-dying method was applied to determine the volumes of cerebral infarctions and nerve function grades scoring to estimate the behaviour of the model.

RESULTS:

TIC-dying method showed that the ischemia area was mainly located in the cortex 2-4 mm behind the optic chiasm. There were no obvious differences among the three groups in the volumes of cerebral infarction, the volumes of cerebral infarctions were larger in the 120 and 180 minute groups than the 90 minute group. The behaviour change of reperfusion after 3 to 6 hours was the most significant in the three groups.

CONCLUSION:

Cerebral ischemia for 120 minutes is ideal for establishing the animal model.

PMID:
16114555
[Indexed for MEDLINE]
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