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EMBO Rep. 2005 Sep;6(9):843-7.

The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis.

Author information

1
Section of Microbial Pathogenesis and Department of Cell Biology, Yale University School of Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA.

Abstract

Ca2+-regulated exocytosis of lysosomes was previously shown to be required for the repair of plasma membrane wounds. The small chemical vacuolin-1 alters the morphology of lysosomes without affecting the ability of cells to reseal their plasma membrane after injury. On the basis of a failure to detect Ca2+-triggered lysosomal exocytosis in vacuolin-1-treated cells, a recent study proposed that lysosomes are dispensable for resealing. Here, we show that vacuolin-1, despite altering lysosome morphology, does not inhibit the exocytosis of lysosomes induced by exposure to a Ca2+ ionophore, or by plasma membrane wounding. Thus, lysosomes cannot be excluded as agents of membrane repair in vacuolin-1-treated cells.

PMID:
16113649
PMCID:
PMC1369166
DOI:
10.1038/sj.embor.7400495
[Indexed for MEDLINE]
Free PMC Article

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