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Biochim Biophys Acta. 2005 Aug 31;1752(1):1-5.

The protein composition of honeybee venom reconsidered by a proteomic approach.

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  • 1Laboratory of Zoophysiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.


Pure honeybee venom samples were submitted to two-dimensional gel electrophoresis. A total of 49 excised spots were analyzed by mass spectrometry; 39 of them resulted in the identification of 6 different known bee venom proteins and of 3 proteins that have not been described in such samples before. The first new venom protein has a platelet-derived and vascular endothelial growth factor family domain, the second protein shows no homologies with any known protein and the third matches a hypothetical protein similar to major royal jelly protein 8.

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