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Int J Tuberc Lung Dis. 2005 Aug;9(8):901-6.

Multicenter study of MTT and resazurin assays for testing susceptibility to first-line anti-tuberculosis drugs.

Author information

1
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. amartin@itg.be

Abstract

OBJECTIVE:

A multicentre evaluation was performed to assess two rapid low-cost methods, MTT (3-[4.5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide) and resazurin assays, for testing the susceptibility of Mycobacterium tuberculosis to the first-line anti-tuberculosis drugs rifampicin (RMP), isoniazid (INH), ethambutol (EMB) and streptomycin (SM).

METHODS:

Thirty coded M. tuberculosis strains were sent to seven laboratories located in Latin America, representing six countries. Each site performed the colorimetric assays, MTT and resazurin, blind for the first-line drugs RMP, INH, EMB and SM. The minimum inhibitory concentration results obtained were compared to the conventional proportion method on Lowenstein-Jensen medium.

RESULTS:

After establishing the breakpoint concentrations, excellent results were obtained for RMP, INH and EMB, with levels of specificity and sensitivity of between 96% and 99%.

CONCLUSION:

MTT and resazurin assays are promising, accessible new alternative methods for middle- and low-resource countries that need low-cost methods to perform rapid susceptibility testing of M. tuberculosis to key anti-tuberculosis drugs.

PMID:
16104638
[Indexed for MEDLINE]

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