Voice-overs are professional voice users who use their voices to market products in the electronic media. The purposes of this study were to (1) analyze voice-overed and non-overed productions of an advertising text in two groups consisting of 10 male professional voice-overs and 10 male non-voice-overs; and (2) determine specific acoustic features of voice-over productions in both groups. A naïve group of listeners were engaged for the perceptual analysis of the recorded advertising text. The voice-overed production samples from both groups were submitted for analysis of acoustic and temporal features. The following parameters were analyzed: (1) the total text length, (2) the length of the three emphatic pauses, (3) values of the mean, (4) minimum, (5) maximum fundamental frequency, and (6) the semitone range. The majority of voice-overs and non-voice-overs were correctly identified by the listeners in both productions. However voice-overs were more consistently correctly identified than non-voice-overs. The total text length was greater for voice-overs. The pause time distribution was statistically more homogeneous for the voice-overs. The acoustic analysis indicated that the voice-overs had lower values of mean, minimum, and maximum fundamental frequency and a greater range of semitones. The voice-overs carry the voice-overed production features to their non-voice-overed production.