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FEMS Microbiol Lett. 2005 Sep 15;250(2):263-9.

Secreted autotransporter toxin produced by a diffusely adhering Escherichia coli strain causes intestinal damage in animal model assays.

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1
Faculdade de Ciências Farmacêuticas, Departamento de Análises, Clinicas e Toxicologicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580 - bl. 17, CEP 05508-900, São Paulo, Brazil.

Abstract

Bacterial diarrheal diseases remain a major cause of morbidity and mortality in developing countries. Diffusely adhering Escherichia coli (DAEC) is a newly proposed category of diarrheagenic E. coli based on epidemiological studies. Sat, a new virulence factor of some uropathogeic Escherichia coli, was described with a vacuolating cytotoxic action in bladder and kidney tissues. In the present study, we analyzed the Sat effects, produced by a DAEC strain in rabbit ileal intestinal tissue and cultured epithelial cells. We observed enterotoxic activity in rabbit ileum tissues by Ussing chamber assays, a pronounced fluid accumulation in rabbit ileum loops with villous necrosis observed in the histopathologic examination, and morphological changes in monolayer cultures of Y1 adrenal cells. Our results suggest that DAEC strains may be involved in diarrhea.

PMID:
16098687
DOI:
10.1016/j.femsle.2005.07.013
[Indexed for MEDLINE]
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