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Metab Eng. 2005 Jul;7(4):318-27.

Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays.

Author information

1
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., Tokyo 194-8533, Japan.

Abstract

Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes.

PMID:
16095938
DOI:
10.1016/j.ymben.2005.06.004
[Indexed for MEDLINE]

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