Format

Send to

Choose Destination
Nat Methods. 2005 Aug;2(8):587-9.

Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides.

Author information

1
Department of Veterinary Preclinical Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZJ, UK. r.beynon@liv.ac.uk

Abstract

Absolute quantification in proteomics usually involves simultaneous determination of representative proteolytic peptides and stable isotope-labeled analogs. The principal limitation to widespread implementation of this approach is the availability of standard signature peptides in accurately known amounts. We report the successful design and construction of an artificial gene encoding a concatenation of tryptic peptides (QCAT protein) from several chick (Gallus gallus) skeletal muscle proteins and features for quantification and purification.

PMID:
16094383
DOI:
10.1038/nmeth774
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center