Format

Send to

Choose Destination
See comment in PubMed Commons below
Phys Rev E Stat Nonlin Soft Matter Phys. 2005 Jun;71(6 Pt 1):061927. Epub 2005 Jun 29.

Control of relative radiation pressure in optical traps: application to phagocytic membrane binding studies.

Author information

1
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. kress@embl.de

Abstract

We show how to control the relative radiation pressure and thereby the stable trap position of an optically trapped bead by variation of the mean incident axial photon momentum. The thermal position fluctuations of a trapped bead are recorded by a three-dimensional back-focal-plane interferometry. The interferometric detection signals are in agreement with predictions based on an extended Mie theory. Depending on the application, the unique and linear range of such a detection system can be optimized by controlling the trap position of the bead. We use this method to investigate in three dimensions the binding of beads to membranes of living cells during phagocytosis. We found that independent of the bead coating (IgG, complement, LPS, avidin) the most frequent initial mechanical response of the cell was a downward pulling of the bead into the cell. The time delay between binding and response was on average 2 s.

PMID:
16089785
DOI:
10.1103/PhysRevE.71.061927
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center