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Cancer Lett. 2006 Jul 8;238(1):53-60. Epub 2005 Aug 1.

Induction of apoptosis by puerarin in colon cancer HT-29 cells.

Author information

1
School of Public Health, Zhengzhou University, Zhengzhou 450052, China. yuzengli@263.net

Abstract

Puerarin was isolated from Pueraria radix and has beneficial effects on cardiovascular, neurological, and hyperglycemic disorders. The current study showed that puerarin also possessed anti-cancer properties. Methyl thiazolyl tetrazolium assay (MTT) assay revealed a dose-dependent reduction of HT-29 cellular growth in response to puerarin treatment. Apoptosis was observed following treatments ;with >or=25 microM puerarin, as reflected by the appearance of the subdiploid fraction and NDA fragmentations. We then investigated effects of puerarin on expression of apoptosis-associated genes and the results revealed an increase of bax and decreases of c-myc and bcl-2. Finally, puerarin treatment significantly increased the activation of caspase-3, a key executioner of apoptosis. These findings indicate that puerarin may act as a chemopreventive and/or chemotherapeutic agent in colon cancer cells by reducing cell viability and inducing apoptosis.

PMID:
16055262
DOI:
10.1016/j.canlet.2005.06.022
[Indexed for MEDLINE]

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