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HHV-8 and EBV are not commonly found in idiopathic pulmonary fibrosis.

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1
Department of Pathology, University of Verona, Italy.

Abstract

BACKGROUND AND AIM OF THE WORK:

Idiopathic Pulmonary Fibrosis/Usual Interstitial Pneumonia (IPF/UIP) is a diffuse and progressive lung disease whose pathogenesis is incompletely understood. Recently, the presence of Herpesvirus-specific DNA was detected in the large majority of cases of a series of IPF/UIP and other lung interstitial diseases. We have therefore tested our own IPF/UIP series for the presence of HHV-8 and EBV proteins.

METHODS:

We used a variety of sensitive technologies including immunohistochemistry with NCL-HHV8-LNA and EBV-LMP1 antibodies, as well as of EBV RNA (EBER) by in-situ hybridisation; the presence of HHV-8 and EBV DNA was also investigated by means of PCR and subsequent analysis using a microfluidic apparatus.

RESULTS:

Despite the good sample quality, immunohistochemical, in-situ hybridisation and PCR results were negative for both EBV and HHV-8.

CONCLUSIONS:

We conclude that EBV and HHV-8 are not involved in the pathogenesis of IPF/UIP.

PMID:
16053027
[Indexed for MEDLINE]
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