Development and validation of a high performance liquid chromatography assay for 17alpha-methyltestosterone in fish feed

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Sep 25;824(1-2):107-15. doi: 10.1016/j.jchromb.2005.07.005.

Abstract

17alpha-Methyltestosterone (MT) is used to manipulate the gender of a variety of fish species. A high performance liquid chromatography (HPLC) internal standard method for the determination of 17alpha-methyltestosterone in fish feed using 3beta-methoxy-17beta-hydroxyandrost-5-en-7-one as internal standard (IS) has been developed. The method has been validated for the quantitation of MT in fish feed using 245 nm UV absorbance as the parent wavelength and 255 nm as a qualifier wavelength. The method was validated in the concentration range of 15.0-120 mg/kg of 17alpha-methyltestosterone in fish feed. Method was also found to be suitable for other feeds.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animal Feed / analysis*
  • Animals
  • Cats
  • Chromatography, High Pressure Liquid / methods*
  • Fishes
  • Methyltestosterone / analysis*
  • Methyltestosterone / standards
  • Rats
  • Reference Standards
  • Reproducibility of Results

Substances

  • Methyltestosterone