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Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):10952-7. Epub 2005 Jul 25.

Cohesins are required for meiotic DNA breakage and recombination in Schizosaccharomyces pombe.

Author information

1
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, A1-162, Seattle, WA 98109, USA.

Abstract

In preparation for the unique segregation of homologs at the first meiotic division, chromosomes undergo dramatic changes. The meiosis-specific sister chromatid cohesins Rec8 and Rec11 of Schizosaccharomyces pombe are recruited around the time of premeiotic replication, and Rec10, a component of meiosis-specific linear elements, is subsequently added. Here we report that Rec10 is essential for meiosis-specific DNA breakage by Rec12 (Spo11 homolog) and for meiotic recombination. DNA breakage and recombination also depend on the Rec8 and Rec11 cohesins, strictly in some genomic intervals but less so in others. Thus, in addition to their previously recognized role in meiotic chromosome segregation, cohesins have a direct role, as do linear element components, in meiotic recombination by enabling double-strand DNA break formation by Rec12. Our results reveal a pathway, whose regulation is significantly different from that in the distantly related yeast Saccharomyces cerevisiae, for meiosis-specific chromosome differentiation and high-frequency recombination.

PMID:
16043696
PMCID:
PMC1182449
DOI:
10.1073/pnas.0504805102
[Indexed for MEDLINE]
Free PMC Article

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