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Biochem Biophys Res Commun. 2005 Sep 9;334(4):1317-21.

Protease-activated quantum dot probes.

Author information

1
Rice University, Department of Bioengineering MS-142, P.O. Box 1892, Houston, TX 77251-1892, USA.

Abstract

We have developed a novel nanoparticulate luminescent probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This construct may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Release of AuNPs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 h of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

PMID:
16039606
DOI:
10.1016/j.bbrc.2005.07.028
[Indexed for MEDLINE]

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