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Am J Ophthalmol. 2005 Jul;140(1):132-4.

Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro.

Author information

1
Department of Ophthalmology, University of Texas Health Science Center, San Antonio, 78229, USA. robertmelendez@mac.com

Abstract

PURPOSE:

The purpose of this study was to evaluate the toxicity and photodynamic activity of indocyanine green (ICG) and trypan blue (TryB) on cultured human lensepithelial cells (LECs).

DESIGN:

Experimental study.

METHODS:

Lens epithelial cell viability was assessed after treatment with ICG and TryB concentrations ranging from 0.025 to 5.0 mg/ml, and exposure to 806 nm diode laser.

RESULTS:

At ICG concentrations below 0.5 mg/ml, there was > or =75% cell viability; at higher ICG concentrations there was dose-dependent cytotoxicity in addition to loss of cellular viability due to ICG photosensitization. TryB had little cytotoxicity to the LECs: >80% cells were viable irrespective of the dye concentration or laser treatment.

CONCLUSIONS:

These data indicate that ICG may have application as a photosensitizer in the selective eradication of residual LECs after cataract surgery to reduce the incidence of posterior capsule opacification.

PMID:
16038657
DOI:
10.1016/j.ajo.2004.12.086
[Indexed for MEDLINE]

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