Format

Send to

Choose Destination
Acc Chem Res. 2005 Jul;38(7):542-8.

Surfaces and orientations: much to FRET about?

Author information

1
Physics Department and Center for Biophysics and Computational Biology, University of Illinois, Urbana-Champaign, Urbana, Illinois 61801, USA.

Abstract

Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.

PMID:
16028888
DOI:
10.1021/ar040138c
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center