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Microsc Res Tech. 2005 May;67(1):36-44.

Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy.

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1
Institute of Photonics, Wolfson Centre, Glasgow G4 0NW, United Kingdom. a.wright@strath.ac.uk

Abstract

We report on the introduction of active optical elements into confocal and multiphoton microscopes in order to reduce the sample-induced aberration. Using a flexible membrane mirror as the active element, the beam entering the rear of the microscope objective is altered to produce the smallest point spread function once it is brought to a focus inside the sample. The conventional approach to adaptive optics, commonly used in astronomy, is to utilise a wavefront sensor to determine the required mirror shape. We have developed a technique that uses optimisation algorithms to improve the returned signal without the use of a wavefront sensor. We have investigated a number of possible optimisation methods, covering hill climbing, genetic algorithms, and more random search methods. The system has demonstrated a significant enhancement in the axial resolution of a confocal microscope when imaging at depth within a sample. We discuss the trade-offs of the various approaches adopted, comparing speed with resolution enhancement.

PMID:
16025475
DOI:
10.1002/jemt.20178
[Indexed for MEDLINE]
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