Characterization of transcription factors and cis-acting elements that regulate human CTP: phosphoethanolamine cytidylyltransferase (Pcyt2)

Christa M Johnson; Zongfei Yuan; Marica Bakovic

doi:10.1016/j.bbalip.2005.06.002

Characterization of transcription factors and cis-acting elements that regulate human CTP: phosphoethanolamine cytidylyltransferase (Pcyt2)

Biochim Biophys Acta. 2005 Aug 15;1735(3):230-5. doi: 10.1016/j.bbalip.2005.06.002.

Authors

Christa M Johnson¹, Zongfei Yuan, Marica Bakovic

Affiliation

¹ Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada N1G 2W1.

PMID: 16023412
DOI: 10.1016/j.bbalip.2005.06.002

Abstract

CTP: phosphoethanolamine cytidylyltransferase (Pcyt2) promoter was isolated from human breast cancer MCF-7 cells and its activity delineated by luciferase reporter assays and gel-shift analysis. The Pcyt2 promoter is driven by a functional CAAT box (-90/-73) and by negative (-385/-255) and positive regulatory elements (-255/-153) in the upstream regions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cloning, Molecular
Gene Expression Regulation, Enzymologic*
Humans
Molecular Sequence Data
Promoter Regions, Genetic
RNA Nucleotidyltransferases / genetics*
RNA Nucleotidyltransferases / metabolism
Recombinant Fusion Proteins / metabolism
Recombinant Proteins / metabolism
Regulatory Sequences, Nucleic Acid
Sequence Deletion
Transcription Factors / metabolism*
Transcription, Genetic

Substances

Recombinant Fusion Proteins
Recombinant Proteins
Transcription Factors
RNA Nucleotidyltransferases
Ethanolamine-phosphate cytidylyltransferase