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Curr Eye Res. 2005 Jul;30(7):543-54.

The mechanisms of cell membrane resealing in rabbit corneal epithelial cells.

Author information

1
Department of Genetics, Development and Cellular Biology, Iowa State University, Ames, Iowa, USA.

Abstract

PURPOSE:

To examine membrane repair mechanisms in rabbit corneal epithelial (RCE) cells.

METHODS:

Microneedle puncture and fluorescent dye loss were used to wound membranes and assay resealing, respectively. Different repair mechanisms were detected pharmacologically and with antisense oligonucleotides.

RESULTS:

The RCE cells rapidly reseal plasma membranes by calcium-dependent exocytotic mechanisms that exhibit both facilitated and potentiated responses to multiple wounding. The facilitated response was inhibited by specific inhibitors of protein kinase C (PKC) and brefeldin A, and the potentiated response was blocked by inhibitors of cAMP-dependent protein kinase (PKA). Reduction of myosin IIA inhibited the facilitated response, and reduction of IIB inhibited the initial resealing.

CONCLUSIONS:

RCE cells rapidly repair plasma membrane disruptions. At a second wound at the same site, PKC stimulated vesicle formation from the Golgi apparatus, resulting in more rapid membrane resealing for a facilitated response. The RCE cell also contains a PKA-dependent global potentiation of membrane resealing.

PMID:
16020288
DOI:
10.1080/02713680590968574
[Indexed for MEDLINE]

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