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Gene. 1992 May 15;114(2):187-93.

The rough (ro+) gene as a dominant P-element marker in germ line transformation of Drosophila melanogaster.

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CSIRO, Division of Biomolecular Engineering, North Ryde, NSW, Australia.


We describe a new vector for the P-element-mediated introduction of gene constructs into the germ line of Drosophila melanogaster. The P-element vector carries 6.8 kb of genomic DNA containing the rough gene (ro) from D. melanogaster and a polylinker (MCS) containing ten unique cloning sites. To demonstrate its utility, we have cloned into the MCS of this vector, the firefly luciferase (Luc)-encoding gene (luc) under the control of the D. melanogaster hsp70 promoter and have transformed flies with the resultant P-element. Single insertions of this element, whether in the hemizygous or homozygous condition, completely rescued the ro- mutation and directed heat-inducible synthesis of Luc mRNA and enzyme.

[Indexed for MEDLINE]

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