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J Antimicrob Chemother. 2005 Sep;56(3):481-4. Epub 2005 Jul 8.

Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae.

Author information

1
School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia. Stephen.Tristram@utas.edu.au

Abstract

OBJECTIVES:

To determine if the TEM beta-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters.

METHODS:

Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the bla(TEM) genes of a collection of TEM-positive strains.

RESULTS:

All bla(TEM) genes were found to be bla(TEM-1) or derivatives thereof and none bla(TEM-2). The bla(TEM) genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution.

CONCLUSIONS:

The genetic features of bla(TEM) genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized.

PMID:
16006445
DOI:
10.1093/jac/dki238
[Indexed for MEDLINE]

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