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Biochem Biophys Res Commun. 2005 Aug 26;334(2):433-40.

A novel methyltransferase required for the formation of the hypermodified nucleoside wybutosine in eucaryotic tRNA.

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Department of Chemistry and Biochemistry, UCLA, Los Angeles, CA 90095-1560, USA.


We demonstrate that the product of the yeast open reading frame YML005w is required for wybutosine (yW) formation in the phenylalanine-accepting tRNA of the yeast Saccharomyces cerevisiae. tRNA isolated from a deletion mutant of the YML005w gene accumulates 4-demethylwyosine (ImG-14), a precursor lacking three of the methyl groups of the yW hypermodified base. Since the amino acid sequence of the YML005w gene contains the signature motifs of the seven beta-strand methyltransferases, we now designate the gene TRM12 for tRNA methyltransferase. Using pulse-chase labeling of intact yeast cells with S-adenosyl-L-[methyl-(3)H]methionine, we show that the methylesterified form of yW is metabolically stable.

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