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Expert Rev Proteomics. 2005 Jun;2(3):295-306.

Complex proteome prefractionation using microscale solution isoelectrofocusing.

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1
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA. tangh@wistar.org

Abstract

The proteomes of mammalian cells, tissues and biologic fluids are complex and consist of proteins present over a wide dynamic range. Current protein profiling technologies do not have the capacity to overcome the sample complexity for comprehensive analysis of complex proteomes. A common strategy to substantially expand protein profiling capacities is sample prefractionation. A prefractionation method developed in the authors' laboratory, microscale solution isoelectrofocusing, has resulted in a commercial product, the ZOOM IEF Fractionator, which provides a simple and convenient method for high-resolution separation of complex proteomes based upon their isoelectric points. Complex human samples such as cancer cells and biologic fluids can be fractionated into well-resolved fractions with minimal cross-contamination of proteins between adjacent fractions. This review focuses on the application of microscale solution isoelectrofocusing prefractionation and subsequent downstream strategies in expanding protein profiling capacities and mining low-abundance proteins of complex proteomes.

PMID:
16000077
DOI:
10.1586/14789450.2.3.295
[Indexed for MEDLINE]
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