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Genome Biol. 2005;6(7):R61. Epub 2005 Jun 30.

Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray.

Author information

1
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, 333 Cassell Drive, Baltimore, MD 21224, USA. cartermar@mail.nih.gov

Abstract

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.

PMID:
15998450
PMCID:
PMC1175992
DOI:
10.1186/gb-2005-6-7-r61
[Indexed for MEDLINE]
Free PMC Article

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