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Biochim Biophys Acta. 2005 Aug 30;1725(1):57-63.

Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts.

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Department of Reproductive Biology and Pathology, National Institute for Child Health and Development, Setagaya-ku, Tokyo 157-8535, Japan.


Several stromal cells were established from murine bone marrow cultures. One of the KUSA subclones, KUSA-A1 cells, displays osteogenic characteristics in vitro and in vivo. The calcium deposition, osteocalcin release, and parathyroid hormone (PTH) responsiveness of KUSA-A1 cells indicate that they are mature osteoblasts or osteocytes. Bone had formed in subcutaneous tissue 1 week after subcutaneous injection of cells into immunodeficient mice. The osteogenesis by KUSA-A1 was not mediated by chondrogenesis and thus was considered to be membranous ossification. These unique characteristics of KUSA-A1 cells provide an opportunity to analyze the process of membranous ossification in detail.

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