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J Bacteriol. 2005 Jul;187(14):4813-21.

Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.

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1
Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Higashimura-cho, Hiroshima, Japan.

Abstract

During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the transcription initiation base determined on primer extension analysis of glsA-glnT) in which a direct repeat, TTTTGTN4TTTTGT, is present. Furthermore, the glsA and glnT genes were biochemically verified to encode glutaminase and glutamine transporter, respectively.

PMID:
15995196
PMCID:
PMC1169493
DOI:
10.1128/JB.187.14.4813-4821.2005
[Indexed for MEDLINE]
Free PMC Article
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