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Foodborne Pathog Dis. 2004 Winter;1(4):258-66.

Detection of viable Mycobacterium avium subsp. paratuberculosis using luciferase reporter systems.

Author information

1
Food Safety Laboratory, Department of Food Science, Cornell University, Ithaca, New York 14853, USA.

Abstract

Plasmid- and phage-based firefly luciferase reporter constructs were evaluated as rapid detection systems for viable Mycobacterium avium subsp. paratuberculosis (MAP). A MAP strain bearing a luciferase-encoding plasmid was detectable at 100 cells/mL in skim milk and 1000 cells/mL in whole milk. Three luciferase-encoding mycobacteriophage were evaluated for detection of wild-type MAP. The best of these, phAE85, allowed detection of >1000 cells/mL within 24-48 h. Membrane filtration did not improve the sensitivity of detection for either plasmid or phage reporters. Luciferase reporters show promise for rapid detection of viable MAP.

PMID:
15992288
DOI:
10.1089/fpd.2004.1.258
[Indexed for MEDLINE]

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