Format

Send to

Choose Destination
Nat Struct Mol Biol. 2005 Jul;12(7):626-7. Epub 2005 Jun 26.

A single active-site region for a group II intron.

Author information

1
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th St., New York, New York 10032, USA.

Abstract

Despite the biological importance of self-splicing group II introns, little is known about their structural organization. Synthetic incorporation of site-specific photo-cross-linkers within catalytic domains resulted in functional distance constraints that, when combined with known tertiary interactions, provide a three-dimensional view of the active intron architecture. All functionalities important for both steps of splicing are proximal before the first step, suggestive of a single active-site region for group II intron catalysis.

PMID:
15980867
DOI:
10.1038/nsmb957
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center