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J Microbiol Methods. 2006 Mar;64(3):406-10. Epub 2005 Jun 24.

An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis.

Author information

1
Institute Neurobiology and Molecular Medicine, Molec. Med. Section, National Research Council, Via del Fosso del Cavaliere, 100, 00133, Rome, Italy.

Abstract

The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples.

PMID:
15979747
DOI:
10.1016/j.mimet.2005.05.002
[Indexed for MEDLINE]

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