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J Virol Methods. 1992 May;37(2):109-17.

Detection of simian immunodeficiency virus RNA from infected rhesus macaques by the polymerase chain reaction.

Author information

1
Department of Medical Pharmacology and Toxicology, School of Medicine, University of California, Davis 95616.

Abstract

A rapid method for the detection of simian immunodeficiency virus (SIV) RNA from peripheral blood mononuclear cells (PBMC) of experimentally infected rhesus macaques by the polymerase chain reaction (PCR) is reported. The PCR was carried out with a complementary DNA (cDNA) template using 3 pairs of primers that were designed to anneal to homologous sequences in conserved regions of 3 molecular clones of SIVmac. The specificity of the primers was confirmed by performing the PCR with template DNA from the 3 molecular clones. SIV-specific RNA was detected from 30 and 50 infected PBMC/6.25 x 10(5) PBMC of two animals.

PMID:
1597502
DOI:
10.1016/0166-0934(92)90038-f
[Indexed for MEDLINE]

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