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Nat Methods. 2005 Jul;2(7):503-5.

Multiphoton excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein.

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1
Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, 465 Kajii-cho Hirokoji Kawaramachi, Kamigyo-ku, Kyoto 602-8566, Japan.

Abstract

Noninvasive, straightforward methods to inactivate selected proteins in living cells with high spatiotemporal resolution are needed. Chromophore-assisted laser inactivation (CALI) can be used to photochemically inactivate proteins, but it has several drawbacks, such as procedural complexity and nonspecific photodamage. Here we show that by application of multiphoton excitation to CALI, enhanced green fluorescent protein (EGFP) is an effective chromophore for inactivation of a protein's function without nonspecific photodamage in living mammalian cells.

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PMID:
15973419
DOI:
10.1038/nmeth770
[Indexed for MEDLINE]

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