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Sheng Wu Gong Cheng Xue Bao. 2003 Nov;19(6):668-73.

[Application of Ac/Ds transposon system to genetate marker gene free transgenic plants in rice].

[Article in Chinese]

Author information

1
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hanrgzhou 310029, China.

Abstract

It is critical to generate marker gene free transgenic plants for retransformating or eliminating the potential harmfulness of marker gene and its product. In this study, Ac/Ds transposon system was developed for removal of hpt selection marker gene to obtain marker-free transgenic plants in rice ( Oryza sativa L.). Ds element containing the interesting gene bar was constructed next to the selection marker gene hpt to get Ds-T-DNA. Rice plants were transformed by Agrobacterium tumefaciens EHA105 containing Ac-T-DNA and Ds-T-DNA respectively. Rice plant containing single copy Ac-T-DNA was crossed with plant containing single copy Ds-T-DNA to obtain the F1 plant containing both Ac and Ds elements. F1 plant was self-crossed to produce F2 progeny in which T-DNA insert and transposed Ds element segregated independently. Two plants contained Ds element but no hpt marker gene in total 100 F2 plants. The result indicated that Ac/Ds transposon system could be used as a vector system for generating marker gene free transgenic plants in rice.

PMID:
15971577
[Indexed for MEDLINE]
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