A B220+ CD117+ CD19- hematopoietic progenitor with potent lymphoid and myeloid developmental potential

Eur J Immunol. 2005 Jul;35(7):2019-30. doi: 10.1002/eji.200526318.

Abstract

In this report, we identify in the bone marrow (BM) of normal mice a subpopulation of B220+ CD117+ CD19- NK1.1- cells with potent lymphoid and myeloid developmental potential. These cells represent 0.1-0.2% of nucleated BM cells. By limiting dilution analysis in the presence of the appropriate combination of stromal cells and cytokines, 1 in 5-10 sorted cells formed B cells, 1 in 10-15 formed T cells and 1 in 5-10 generated macrophages. When cultured on a mixture of OP9 stroma and OP9 stromal cells expressing the Notch ligand Delta-like-1, single cells generated both T and B cells. Following intravenous infusion, freshly sorted cells transiently reconstituted both the T and B cell progenitor compartments, generating cohorts of mature T and B lymphocytes. The relationship between B220+ CD117+ CD19- NK1.1- cells of wild-type mice and other multi-lineage BM progenitors is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / immunology
  • Antigens, CD19 / biosynthesis*
  • Antigens, Ly
  • Antigens, Surface
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Cell Differentiation / immunology
  • Female
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology*
  • Lectins, C-Type
  • Leukocyte Common Antigens / biosynthesis*
  • Lymphoid Tissue / cytology
  • Lymphoid Tissue / immunology
  • Mice
  • Mice, Congenic
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Myeloid Cells / cytology
  • Myeloid Cells / immunology
  • NK Cell Lectin-Like Receptor Subfamily B
  • Proteins / immunology
  • Proto-Oncogene Proteins c-kit / biosynthesis*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology

Substances

  • Antigens
  • Antigens, CD19
  • Antigens, Ly
  • Antigens, Surface
  • Klrb1c protein, mouse
  • Lectins, C-Type
  • NK Cell Lectin-Like Receptor Subfamily B
  • Proteins
  • Proto-Oncogene Proteins c-kit
  • Leukocyte Common Antigens