Abstract
In this report, we identify in the bone marrow (BM) of normal mice a subpopulation of B220+ CD117+ CD19- NK1.1- cells with potent lymphoid and myeloid developmental potential. These cells represent 0.1-0.2% of nucleated BM cells. By limiting dilution analysis in the presence of the appropriate combination of stromal cells and cytokines, 1 in 5-10 sorted cells formed B cells, 1 in 10-15 formed T cells and 1 in 5-10 generated macrophages. When cultured on a mixture of OP9 stroma and OP9 stromal cells expressing the Notch ligand Delta-like-1, single cells generated both T and B cells. Following intravenous infusion, freshly sorted cells transiently reconstituted both the T and B cell progenitor compartments, generating cohorts of mature T and B lymphocytes. The relationship between B220+ CD117+ CD19- NK1.1- cells of wild-type mice and other multi-lineage BM progenitors is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens / immunology
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Antigens, CD19 / biosynthesis*
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Antigens, Ly
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Antigens, Surface
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology
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Cell Differentiation / immunology
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Female
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / immunology*
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Lectins, C-Type
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Leukocyte Common Antigens / biosynthesis*
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Lymphoid Tissue / cytology
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Lymphoid Tissue / immunology
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Mice
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Mice, Congenic
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Myeloid Cells / cytology
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Myeloid Cells / immunology
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins / immunology
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Proto-Oncogene Proteins c-kit / biosynthesis*
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
Substances
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Antigens
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Antigens, CD19
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Antigens, Ly
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Antigens, Surface
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Klrb1c protein, mouse
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Lectins, C-Type
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins
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Proto-Oncogene Proteins c-kit
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Leukocyte Common Antigens