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Malar J. 2005 Jun 21;4:26.

Molecular analysis of two local falciparum malaria outbreaks on the French Guiana coast confirms the msp1 B-K1/varD genotype association with severe malaria.

Author information

1
Centre National de Référence sur la Chimiorésistance du Paludisme dans la région Antilles-Guyane, Institut Pasteur de la Guyane, BP 6010, F-97306 Cayenne-Cedex, France. elegrand@pasteur-cayenne.fr

Abstract

BACKGROUND:

Plasmodium falciparum outbreaks can occur in the coastal area of French Guiana, where the population is essentially non-immune. Two sporadic outbreaks were observed, including one with severe malaria cases. To characterize these outbreaks and verify previous observations of specific genotype characteristics in severe malaria in this area, all cases from each outbreak were studied.

METHODS:

P. falciparum genotypes for six genetic loci were determined by PCR amplification from peripheral blood parasites. The msp1/block2 and msp2 genotypes were determined by DNA sequencing. Microsatellite and varD genotyping was based on size polymorphism and locus-specific amplification.

RESULTS:

The outbreak including severe malaria cases was associated with a single genotype. The other mild malaria outbreak was due to at least five distinct genotypes.

CONCLUSION:

Two distinct types of outbreak occurred despite systematic and sustained deployment of malaria control measures, indicating a need for reinforced vigilance. The varD/B-K1 msp1 linkage and its association with severe malaria in this area was confirmed.

PMID:
15969766
PMCID:
PMC1187918
DOI:
10.1186/1475-2875-4-26
[Indexed for MEDLINE]
Free PMC Article

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