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Sheng Wu Gong Cheng Xue Bao. 2003 Jan;19(1):112-5.

[Expression of phytase gene in Bombyx mori].

[Article in Chinese]

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Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Zhenjiang 212018, China.


Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.

[Indexed for MEDLINE]

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