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Sheng Wu Gong Cheng Xue Bao. 2003 Jan;19(1):112-5.

[Expression of phytase gene in Bombyx mori].

[Article in Chinese]

Author information

1
Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Zhenjiang 212018, China.

Abstract

Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.

PMID:
15969047
[Indexed for MEDLINE]

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